Enabling
Advanced
Bioprocessing
Innovative solutions that
challenge the status quo
Capabilities Catalog
Bioprocessing
solutions built to
empower your
success
Since 1981, Repligen has been a
trusted partner to biopharmaceutical
manufacturers, driving progress
through innovative, high-value
bioprocessing solutions.
We understand the challenges you
face—reducing production time,
lowering costs, improving yields,
and ensuring top-tier product
quality. That’s why our cutting-edge
technologies and flexible solutions
are designed to meet your specific
needs at every critical stage of
biologic drug production.
Repligen redefines what is possible
in bioprocessing by delivering
innovative tools and expert guidance
that help you streamline workflows,
accelerate process development,
and achieve optimal results in
both clinical and commercial
manufacturing. Whether you’re
scaling up or fine-tuning, our
solutions are built to empower
your success.
Contents
Modalities
Workflow Capabilities and Solutions
Global Manufacturing Network
XCell® ATF Systems
10
KrosFlo® KPS TFF Systems
12
KrosFlo® TFDF® Systems
14
Upstream Process Analytical Technology
16
Cell Culture Supplements
18
KRM® Chromatography Systems
20
OPUS® Pre-packed Chromatography Columns
22
KrosFlo® KTF + TFF Systems
24
KrosFlo® RS TFF Systems
26
KrosFlo® KR2i, KMPi, and FS TFF Systems
28
KrosFlo® RPM® TFF Systems
30
Ligands and AVIPure® Affinity Resins
32
Spectrum® Hollow Fiber Filters and TangenX® TFF Cassettes
34
Downstream Process Analytical Technology
36
ProConnex® Fluid Management Solutions
38
ProConnex® Single-Use Valve Technologies
40
ProConnex® MixOne CB-X and RG-X Systems
42
Metenova® Stainless Steel Mixers
44
Our Team
46
Sustainability
47
Repligen Enabling Advanced Bioprocessing
Antibodies and proteins
• Monoclonal antibodies: A cornerstone of biologic drug development, mAbs require
scalable, high-yield processes that maintain product quality and consistency of mabs
production.
• Antibody-drug conjugates (ADCs): By combining targeted antibody delivery with
potent cytotoxins, ADCs demand precise upstream and downstream production
control to ensure drug efficacy and require contained, aseptic processing to ensure
operator safety.
• Antibody fragments: Smaller and highly specific, antibody fragments call for flexible
purification and formulation approaches that preserve functionality.
• Bispecific antibodies: With dual-targeting capabilities, bispecifics introduce added
complexity in expression system and purification, requiring highly optimized process
solutions.
• Recombinant proteins: A diverse and rapidly evolving modality, recombinant proteins
benefit from adaptable workflows that support a wide range of molecular formats
and applications.
Viral vectors
• Adeno-associated virus (AAV): A key viral vector in gene therapy, AAV use is
expanding from rare disease applications to more common conditions, requiring
scalable, high-yield production processes that maintain quality while keeping drug
costs affordable.
• Lentivirus: A major viral vector platform used for both in vivo and ex vivo therapies,
lentivirus requires production methods that preserve its shear-sensitive nature while
ensuring consistent, high-yield, and safe products.
Bioprocessing solutions to enable a new
generation of therapeutics
From established biologics to emerging platforms, today’s therapeutic modalities are
transforming disease treatment.
Repligen solutions are designed to help you overcome process development and
manufacturing challenges and support the production of innovative therapies across
a broad range of modalities, powering precision oncology, cell and gene therapies,
and more.
Cell therapy
• Induced pluripotent stem cells, T cells, CAR T-cells, natural killer cells, and
mesenchymal stem cells: In cell therapy, cells must be selected, modified, and
expanded, but the buildup of toxic metabolites can inhibit this process. Integration of
perfusion culture into the bioreactor can help to intensify potent cells and generate
more doses per batch.
• Extracellular vesicles (EV) and exosomes: EVs and exosomes are promising cell-free
therapies, but scaling up their production with consistent quality is challenging.
Perfusion systems help drive efficiency by removing waste, maintaining EV quality,
and concentrating EVs continuously.
Nucleic acid therapeutics
• Messenger RNA (mRNA): mRNA therapeutics demand precise in vitro transcription
processes and effective dsRNA removal to ensure stability and efficacy.
• Plasmid DNA (pDNA): A critical starting material for many advanced therapies, pDNA
production requires high-purity yields and scalable manufacturing workflows.
Whether you’re scaling up an established platform or developing new molecules, Repligen
provides bioprocessing tools, technologies, and support to help you drive progress, improve
process performance, and deliver impactful therapies.
Repligen Enabling Advanced Bioprocessing
Empowering productivity and facility
efficiency in bioprocessing
Repligen products across the bioprocessing workflow
Repligen Enabling Advanced Bioprocessing
Ensure product availability with
manufacturing redundancy and
a secure supply chain
Learn more about our global locations
Corporate Headquarters
(Waltham, MA)
Global network designed to meet our customers’ needs
Headquarters
Waltham, MA (HQ)
Manufacturing Sites - United States
Anaheim, CA
Irving, TX
Auburn, MA
Lebanon, NH
Bridgewater, NJ
Marlborough, MA
Clifton Park, NY
Rancho Dominguez, CA
Hopkinton, MA
Manufacturing Sites - Europe
Breda, The Netherlands
Tallinn, Estonia
Lund, Sweden
Toulouse, France
Mölndal, Sweden
Waterford, Ireland
Ravensburg, Germany
Asia-Pacific Commercial Offices
Bangalore, India
Shiga, Japan
Gyeonggi-do, Korea
Singapore
Shanghai, China
Multi-site
Manufacturing
Safety Stock
Management Program
Secure Raw Material
Supply Chains
Complete Regulatory
Support Packages
Hands-on Service
10
Repligen Enabling Advanced Bioprocessing
Increase yield and improve efficiency
with process intensification
XCell® ATF Systems
Learn more about XCell® ATF Systems
XCell® ATF Systems enable continuous, intensified bioprocessing using Alternating Tangential Flow
(ATF) technology.
• XCell® ATF Devices use alternating bidirectional flow that generates a backflush action that
cleans the filter, minimizing clogging and fouling.
• XCell® ATF Controllers provide precise control, maintaining flow rates and monitoring filter
health to enable up to 60 days of continuous perfusion without filter changeout.
Intensified processes using XCell® ATF Systems optimize space-time yield, achieving higher viable
cell densities in smaller bioreactors, reducing facility footprint, and significantly increasing overall
productivity and yield from process scale to commercial scale.
11
UPSTREAM
Why process intensification?
Process intensification improves upstream biomanufacturing by boosting
productivity and reducing cost of goods. Compared to fed-batch, perfusion
processes with XCell® ATF Technology deliver 5–10X greater volumetric
productivity, shorten production timelines by 15–30 days, and reduce upstream
cost of goods by up to 24%1.
1. Repligen (2025) From Fed-Batch to Perfusion: Unlocking ROI and Capacity in Monoclonal Antibody
Manufacturing. [White paper] BioProcess International.
12
Repligen Enabling Advanced Bioprocessing
Enable high-density cell culture with
TFF-based perfusion
KrosFlo® KPS Tangential Flow Filtration (TFF) Systems
KrosFlo® KPS Systems These systems
are engineered for cell culture
perfusion operations, delivering a
broad range of flow rates, robust
automation, and single-use flexibility
for cGMP manufacturing. Built on
decades of TFF expertise, KrosFlo®
KPS Systems provide an economical
solution that combines gentle
processing, a wide range of filter
options, and scalable performance
within intensified upstream workflows.
To unlock the full potential of the
KrosFlo® KPS System, pair it with
Spectrum® Hollow Fiber Filters—
purpose-built for reliable cell retention
in upstream perfusion. Engineered for
seamless integration, Spectrum® filters
deliver exceptional viability, low-shear
handling, and consistent performance
even at high cell densities. Together,
they form a robust, closed-loop TFF
solution that supports high product
yield, and scalable operation from
development through production.
13
UPSTREAM
14
Repligen Enabling Advanced Bioprocessing
Simplify and intensify
viral vector bioprocessing
KrosFlo® TFDF® Systems
Learn more about KrosFlo® TFDF® Systems
KrosFlo® TFDF® Technology
The KrosFlo® Tangential Flow Depth
Filtration® (TFDF®) Systems simplify and
improve viral vector bioproduction by
increasing viable cell density, productivity,
yield, and recovery for a more robust and
efficient process. This game-changing
technology combines the benefits of
tangential flow filtration (TFF) with the
efficiency of depth filtration (DF) to deliver
a scalable, high-performance solution for
applications such as perfusion, continuous
viral particle harvest, and clarification.
ProConnex® TFDF® Flow Paths
ProConnex® TFDF® Flow Paths are ready-
to-use, closed single-use assemblies
that arrive gamma-irradiated, dry, and
preservative-free. Requiring zero flushing,
each assembly includes a TFDF® filter, a
disposable pump head, integrated sensors,
tubing, and aseptic connectors, ensuring
seamless set-up and sterile operation.
15
UPSTREAM
16
Repligen Enabling Advanced Bioprocessing
Accelerate your upstream with
PATsmart™ Process Analytical Solutions
At-line media profiling and in-line key process
parameters analysis
Learn more about upstream PAT solutions
PATsmart™ MAVEN® System
Take command of your cell culture process with the
PATsmart™ MAVEN® Online Glucose and Lactate
Monitoring System. Delivering continuous, sample-
free glucose data every 2 minutes the MAVEN®
automates feeding strategies, reduces variability, and
frees your team from manual sampling. Designed to
integrate seamlessly into bioreactor operations, the
MAVEN® brings precision and efficiency to every run
without disrupting your workflow.
PATsmart™ MAVERICK® Analyzer
The PATsmart™ MAVERICK® Bioprocess
Analyzer is an in-line, plug-and-play,
Raman-based PAT system that provides
real-time, minute-to-minute measurements
of multiple key bioprocess parameters,
including glucose, lactate, and total biomass,
without the drain of empirical modeling,
reagents, or sample extraction on your
time and resources. Ideal for monitoring
metabolic health, guiding dynamic feed
control, and determining optimal harvest
times, the MAVERICK® can even oversee
multiple bioreactors with just one system,
all while it interprets the data for you.
17
UPSTREAM
PATsmart™ ZipChip® Interface
The PATsmartTM ZipChip® Microfluidic CE-
MS Interface delivers rapid, high-resolution
separations by integrating capillary electrophoresis
with electrospray ionization for direct mass
spectrometry. With minimal prep—just load and
run—it enables fast, efficient characterization of
proteins, metabolites, oligonucleotides, and small
molecules in just minutes.
PATsmart™ REBEL® XT System
Built on the proven foundation of the award-winning
PATsmart™ REBEL®analyzer, the PATsmart™ REBEL® XT
System continues to challenge the limits of traditional lab
testing, by delivering rapid, at-line amino acid and choline
profiling using capillary electrophoresis-mass spectrometry
(CE-MS). With streamlined sample prep, the REBEL® XT
system makes sure you know your sample inside and out,
by delivering actionable data on 22 analytes, all in just
15 minutes.
18
Repligen Enabling Advanced Bioprocessing
Learn more about our powerful growth factors
Optimize cell culture
growth and productivity
LONG® R3 IGF-I and LONG® EGF
Cell Culture Supplements
Optimizing cell culture growth and productivity is key to cell culture expansion.
Recombinant growth factors reduce stress, support long-term growth and
enhance the productivity of chemically-defined media.
LONG® R3 IGF-I is a recombinant analog of human
insulin-like growth factor-I (IGF-I) engineered to
boost cell culture performance.
• Higher potency than insulin or native IGF-I.
• Optimized for serum-free and low-serum media.
• Supports higher cell density viability, and longer
culture run.
LONG® EGF is a recombinant analog of human
epidermal growth factor (EGF) that activates the
ErbB-1 receptor to drive robust cell growth and
productivity. An ideal replacement for native or
recombinant EGF.
• Supports cell growth & viability of therapeutic
cell culture applications
• Boosts productivity in a wide range of cell lines
• Optimized for serum-free and low-serum media
19
UPSTREAM
LONG® R3 IGF-I ELISA Kit. The only commercially available kit for the quantitation of LONG® R³
IGF-I, the kit is fast, accurate, and easy-to-use with both cell culture and drug substance samples.
The sandwich ELISA kit uses a biotinylated secondary antibody and streptavidin-HRP for detection.
• Limited reactivity to native or recombinant IGF-I
• No reactivity to insulin
• Ready-to-use ELISA plate allows the assay to be run immediately without additional plate
coating and blocking steps
Learn more about ELISA kits
20
Repligen Enabling Advanced Bioprocessing
Improve yield and increase
efficiency with a single-use
chromatography platform
KRM® Chromatography Systems
Learn more about KRM® Chromatography Systems
KRM® Chromatography Systems are scalable, single-use platforms that use
advanced hardware engineering, fluid management, and process control to
enhance scalability, improve recovery, and accelerate batch changes, thereby
improving facility efficiency.
• Scalable across four
system sizes, the platform
supports flow rates from
1 to 3600 L/h with a single
optimized flow path design,
consistent hardware, and a
uniform user experience for
seamless scale-up.
• Engineered to minimize
hold-up volume and
cross-contamination and
to ensure precise buffer
control for maximal product
recovery.
• User-centered design
reduces human error,
minimizes contamination
risk, and lowers downtime
for safer, more efficient
workflows.
• KlariFi™ software simplifies
automation, enhances
process control, and helps
customers unlock the full
potential of their workflow.